TTML FEE STRUCTURE

TO PLACE ORDER (items & services)

TO SCHEDULE EQUIPMENT (COMING SOON!)

Media orders require 3 BUSINESS DAYS’ notice

* Please acknowledge the TTML in your publications (see Nature 2024) *

REPOSITORY BANK of well-characterized primary human cell lines

COMING SOON… equipment time for (1) ECIS continuous TEER measurement, (2) EVOM Manual TEER, (3) Multisizer 4e Cell Counter, (4) Synergy H1MG with dual injectors, and (5) gentleMACS Dissociator with Heaters

LWRN-conditioned Medium

• Generated from ATCC cell line CRL-3276 according to Miyoshi et al. (Nat. Protocols, 2013)

• This media is not ready-to-use. See Protocols for generating complete medium: Human Colonoid Medium.

• Quality Control: TopFlash luciferase reporter or One-Glo assay to determine WNT signaling activity, mycoplasma testing, and functional testing on colonoid lines. Details available upon request (representative QC data). 

Human Colonoid Medium (HCM-Y)

• HCM-Y is ready-to-use. Made with LWRN-conditioned Media plus a host of other supplements/factors (full formulation). 

• HCM-Y has been used to successfully grow epithelium-only stomach, small intestine and colonic organoids from adult human tissue.

*Comparing Laboratory Prepared Medium and a Commercial Medium Alternative for Expansion of Human Colonoid Cultures.

Human Intestinal Epithelium as 3D Organoids and 2D Monolayer/Transwell

Prior to transfer of enteroids/colonoids to University of Michigan investigators, a Simple Letter Agreement is required. This includes cell line transfers and ready-to-use 3D and 2D formats.