TTML FEE STRUCTURE

TO PLACE ORDERS

Media orders require 3 BUSINESS DAYS’ notice

* Please acknowledge the TTML in your publications (see Nature 2024) *

REPOSITORY BANK of well-characterized primary human cell lines

LWRN-conditioned Medium

• Generated from ATCC cell line CRL-3276 according to Miyoshi et al. (Nat. Protocols, 2013)

• This media is not ready-to-use. See Protocols for generating complete medium: Human Colonoid Medium.

• Quality Control: TopFlash luciferase reporter or One-Glo assay to determine WNT signaling activity, mycoplasma testing, and functional testing on colonoid lines. Details available upon request (representative QC data). 

Human Colonoid Medium (HCM-Y)

• HCM-Y is ready-to-use. Made with LWRN-conditioned Media plus a host of other supplements/factors (full formulation). 

• HCM-Y has been used to successfully grow epithelium-only stomach, small intestine and colonic organoids from adult human tissue.

*Comparing Laboratory Prepared Medium and a Commercial Medium Alternative for Expansion of Human Colonoid Cultures.

Human Intestinal Epithelium as 3D Organoids and 2D Monolayer/Transwell

Prior to transfer of enteroids/colonoids to University of Michigan investigators, a Simple Letter Agreement is required. This includes cell line transfers and ready-to-use 3D and 2D formats.